It depends on how often Google visits your site. Check out you stats in Google webmaster tools.
You can change them a million time and if Google visits on the last time they will only think they changed once.
Changing Title tags can have an adverse affect on your rankings if you are not careful about where you rank for the keywords in your title tag.
If you know where you rank for a certain keyword and change it to benefit this keyword then you're ok. But if you have no idea you rank high for the ketwords in you title tags and go and change them, you will now loose out.
That's why it's so importan to know ALL the keywords people are using to reach your site and where you are in that search engine.
I'm telling you all: use hittail.com and this will be more of a benefit than you could ever imagine. After a few days you will start seeing exactly how to change your title tags to better improve your rankings.
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